¶ Huygens Pro
You'll find here some info reagarding the usage of the HuygensPro software.
¶ Create a PSF
¶ Open an image of beads
Please check the parameters of your image, going in Edit mic
including voxel size, microscopy acquisition type, wavelength
¶ Average Spheres
Select your image, right-click > Operation Window, select Avg Spheres, specify the destination image, then Run Command
¶ Create a PSF
Select the "Average image", right-clic > Operation WIndow , Distill PSF, specify the destination image, then Run Command
¶ Deconvolve using your PSF
Using your generated psf
Select your image, right-clic > Operation WIndow, CMLE,specify the psf and the destination image, then Run Command
